For immunostaining under
1 hour
|
ICAM -1 |
ER |
AE1/AE3 |
KI-67 |
Cyclin
B |
 |
 |
 |
 |
 |
STAT
STAINING SYSTEMS FOR RAPID, NO-WASH, NO-BACKGROUND STAINING
A
single kit system for animal and human tissue staining
About STAT-Q
STAT-Q is a 3-step, amplified biotin-strepavidin system
developed for WASH FREE, BACKGROUND FREE, PRE-TREATMENT
FREE immunostaining in 40 minutes.
UNIQUE FEATURES
•
No serum or protein blocking required
• Total staining time of only 40 minutes for paraffin
sections (from primary to mounted slides).
• Total staining time of 15-20 minutes for frozen
sections
• Wash Free, one 5 second rinse in between incubation
steps
• No Avidin blocking required
• Short 10 minute incubation steps
• Highly amplified, cuts incubation time of 30 minute
primaries to 10 minutes
• Allows at least 2 fold increase in dilutions of
current primary antibodies at 10 minute incubation time
• No re-titration of current primary antibody titer
is required, simply switch to STAT-Q staining system
• Economical: Average cost per slide 65 cents
• 3 years of shelf life
STAT-Q USER FRIENDLY PROTOCOL
TOTAL STAINING TIME: 35-40 minutes
No protein blocking step or extensive rinses are required
when staining with Innovex staining systems.
1. Rinse once with PBS or Innovex “Signal Enhancing
Wash Buffer” for 5 seconds prior to the application
of primary antibody;
2. Incubate the section or smear for 10 minutes with appropriate
primary antibody, rinse once with PBS or Innovex “Signal
Enhancing Wash Buffer” for 5 seconds;
3. Incubate with secondary linking antibody for 10 minutes,
rinse once with PBS or Innovex “Signal Enhancing Wash
Buffer” for 5 seconds;
4. Incubate with enzyme-strepavidin label for 10 minutes,
rinse once with PBS or Innovex Signal Enhancing Wash Buffer”
for 5 seconds;
5. Incubate with INNOVEX substrate/chromogen of choice (AEC
or DAB for HRP enzyme label) and Innovex Brown, Innovex
Faster Red or other substrate/chromogen available for alkaline
phosphatase enzyme for 5-10 minutes;
6. Rinse with water, counterstain and mount.
For background free animal tissue staining, simply, incubate
all species tissues with Innovex Background Buster (Product
# NB306) for 20-30 minutes prior to application of the primary
antibody and proceed to stain as protocol above.
STAT-Q
Peroxidase Multivalent Complete systems (Large
Volume)
(single staining system for staining both
mouse and rabbit primary antibodies) include :
• 60 ml of amplified biotinylated linking antibody
for staining both mouse and rabbit primary antibodies
• 60 ml of stable and polymerized peroxidase (HRP)
labeled strepavidin with no diminishing activity with
time
• 70 ml of stable AEC or DAB of choice.
Large Volume Stains 800-1000 Slides STAT-Q
Peroxidase Multivalent Complete systems
(Small Volume)
• 20 ml of amplified biotinylated linking antibody
for staining both mouse and rabbit primary antibodies
• 20 ml of stable and polymerized peroxidase (HRP) labeled strepavidin with no diminishing activity
with time
• 30 ml of stable AEC or DAB of choice.
Small Volume Stains 200-300 Slides
TYPE
|
NUMBER
|
PRICE |
|
Multivalent-DAB
(LARGE VOLUME) |
NB314KLD |
$645 |
|
Multivalent-AEC
(LARGE VOLUME) |
NB314KLC |
$645 |
|
Multivalent-DAB
(SMALL VOLUME) |
NB314KLD-20 |
$345 |
|
Multivalent-AEC
(SMALL VOLUME) |
NB314KLC-20 |
$345 |
|
STAT-Q
Mouse Adsorbed Kit
For Staining Rat Antibodies on
Mouse Tissue |
NB314MAK-
DAB (15 ml) |
$675 |
|
STAT-Q
Mouse Adsorbed Kit
For Staining Rat Antibodies on
Mouse Tissue |
NB314MAK-
AEC (15 ml) |
$675 |
|
STAT-Q
Rat Adsorbed Kit
For Staining Mouse Antibodies on Rat Tissue |
NB314RAK-
DAB (15 ml) |
$675 |
|
STAT-Q
Rat Adsorbed Kit
For Staining Mouse Antibodies on Rat Tissue |
NB314RAK-
AEC (15 ml) |
$675 |
|
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| |
STAT-Q
Alkaline phosphatase Multivalent Complete systems (Large
Volume)
(single system for staining both mouse and
rabbit primary antibodies) include:
• 60 ml of amplified biotinylated linking antibody
for staining both mouse and rabbit primary antibodies
• 60 ml of stable and amplified alkaline phosphatase
labeled strepavidin
• Substrate/chromogen of choice, Innovex FAST
- RED or Innovex BROWN
Large Volume Stains 800-1000 Slides STAT-Q
Alkaline phosphatase Multivalent Complete systems (Small
Volume)
• 20 ml of amplified biotinylated linking antibody
for staining both mouse and rabbit primary antibodies
• 20 ml of stable and amplified alkaline phosphatase
labeled strepavidin
• Substrate/chromogen of choice, Innovex FAST
- RED or Innovex BROWN
Small Volume Stains 200-300 Slides
TYPE
|
NUMBER
|
PRICE |
|
Multivalent-FAST
RED
(LARGE VOLUME) |
NB311KLF |
$645 |
|
Multivalent-INNOVEX
BROWN
(LARGE VOLUME) |
NB311KLB |
$645 |
|
Multivalent-FAST
RED
(SMALL VOLUME) |
NB311KLF-20 |
$345 |
|
Multivalent-INNOVEX
BROWN
(SMALL VOLUME) |
NB311KLB-20 |
$345 |
|
| |
|
|
|
|
COMPONENTS
STAT-Q staining system components are available in:
|
|
Item# |
Product Description |
Unit |
PriceUs $ |
Buy |
|
NB314KL-60 |
STAT-Q Multivalent Linking antibody, for staining both
MOUSE & RABBIT primary
antibodies |
60 ml |
$
375 |
|
|
NB314KL-20 |
STAT-Q Multivalent Linking antibody, for staining both
MOUSE & RABBIT primary
antibodies |
15 ml |
$
225 |
|
|
NB314KM-20 |
STAT-Q Monovalent-Mouse Linking antibody for staining
MOUSE primary
antibodies |
15 ml |
$
225 |
|
|
NB314KR-20 |
STAT-Q Monovalent-Rabbit Linking antibody for staining
RABBIT primary antibodies |
15 ml |
$
225 |
|
|
NB318LS-20 |
STAT-Q Monovalent-Sheep Linking antibody for staining
SHEEP primary antibodies |
15 ml |
$
225 |
|
|
NB318LG-20 |
STAT-Q Monovalent-Goat Linking antibody for staining
GOAT primary antibodies |
15 ml |
$
225 |
|
|
NB318KH-20 |
STAT-Q Monovalent-Hamster Linking antibody for staining
HAMSTER primary antibodies |
15 ml |
$
225 |
|
|
NB317LC-20 |
STAT-Q Monovalent-Chicken Linking antibody for staining
CHICKEN primary antibodies |
15 ml |
$225 |
|
|
NB318KR-20 |
STAT-Q Monovalent-Rat Linking antibody for staining
RAT primary antibodies |
15 ml |
$225 |
|
|
NB619RK-20 |
STAT-Q Monovalent-Rat Linking antibody MOUSE ADSORBED
for staining
Rat primary antibodies in mouse tissue |
15
ml |
$
295 |
|
|
NB318MR-20 |
STAT-Q Monovalent-Mouse Linking antibody RAT ADSORBED
for staining
MOUSE primary antibodies in Rat tissue |
15 ml |
$
295 |
|
|
STAT-Q
ENZYME LABELED STREPAVIDIN ARE AVAILABLE IN: |
|
NB314L |
Peroxidase (HRP) label (large volume) |
60 ml |
$
395 |
|
|
NB314L-20 |
Peroxidase (HRP) label |
15 ml |
$
225 |
|
|
NB311L-20 |
Alkaline phosphatase-strepavidin label 0 |
15 ml |
$
245 |
|
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