For immunostaining under 1 hour

Please click this icon to download the data sheet in PDF file.

 

STAT STAINING SYSTEMS FOR RAPID, NO-WASH, NO-BACKGROUND STAINING
A single kit system for animal and human tissue staining

About STAT-Q
STAT-Q is a 3-step, amplified biotin-streptavidin system developed for WASH FREE, BACKGROUND FREE, PRE-TREATMENT FREE immunostaining in 40 minutes.

UNIQUE FEATURES
• No serum or protein blocking required
• Total staining time of only 40 minutes for paraffin sections (from primary to mounted slides).
• Total staining time of 15-20 minutes for frozen sections
• Wash Free, one 5 second rinse in between incubation steps
• No Avidin blocking required
• Short 10 minute incubation steps
• Highly amplified, cuts incubation time of 30 minute primaries to 10 minutes
• Allows at least 2 fold increase in dilutions of current primary antibodies at 10 minute incubation time
• No re-titration of current primary antibody titer is required, simply switch to STAT-Q staining system
• Economical: Average cost per slide 65 cents
• 3 years of shelf life


STAT-Q USER FRIENDLY PROTOCOL
TOTAL STAINING TIME: 35-40 minutes
No protein blocking step or extensive rinses are required when staining with Innovex staining systems.

1. Rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds prior to the application of primary antibody;

2. Incubate the section or smear for 10 minutes with appropriate primary antibody, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;

3. Incubate with secondary linking antibody for 10 minutes, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;

4. Incubate with enzyme-streptavidin label for 10 minutes, rinse once with PBS or Innovex Signal Enhancing Wash Buffer” for 5 seconds;

5. Incubate with INNOVEX substrate/chromogen of choice (AEC or DAB for HRP enzyme label) and Innovex Brown, Innovex Faster Red or other substrate/chromogen available for alkaline phosphatase enzyme for 5-10 minutes;

6. Rinse with water, counterstain and mount.

For background free animal tissue staining, simply, incubate all species tissues with Innovex Background Buster (Product # NB306) for 20-30 minutes prior to application of the primary antibody and proceed to stain as protocol above.

 

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