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For IHC staining in 60 minutes
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PAN CYTOKERATIN
HUMAN BREAST CARCINOMA |
bcl-2 |
CYTOKERATIN 7
HUMAN BREAST CARCINOMA |
CD 45
HUMAN TONSIL |
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For Wash- Free, Background-Free STAT (rapid) immunostaining in 2 easy steps and under one hour. Free of avidin, biotin, and streptavidin
A single kit system for animal and human tissue staining
ABOUT POLYMER HISTO-STAT Kits
HISTO-STAT (2-step staining system) is a Non-biotin & Non-streptavidin system developed for wash-free,
background-free IHC stainingin 60 minutes. Does NOT require Protein/ serum Blocking.
Innovex Polymer HIsto-Stat kits only involve 2 staining steps post incubation with the primary antibody;
an incubation with the Polymer reagent and, a short incubation step (5 minutes)
with the substrate/chromogen
of choice.
Histo-stat uses one less reagent, one less
incubation step and one less rinse step.
UNIQUE FEATURES
- No Background. No serum or protein blocking step required
- No re-titration of current primary antibodies required, simply use HISTO-STAT Polymer kits with your current primary antibody titers
- Total staining time of only 60 minutes for paraffin sections and 20-30 minutes for frozen sections
- Wash-Free, only 5 second rinses in between each incubation step.
- Free of biotin; No avidin/ biotin blocking required
- Short incubation steps
- 3 years of shelf life
- Highly amplified, equates the sensitivity of biotin-streptavidin system
HISTO-STAT USER FRIENDLY PROTOCOL (total staining time: 60 minutes for paraffin
section, 20 minutes for frozen sections)
No protein blocking step or extensive rinses are required when staining with Innovex staining systems.
Post Deparaffinization:
- Incubate the section or smear for 20 minutes with primary antibody, rinse once
with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
- Incubate with the Peroxidase (HRP) or alkaline phosphatase Second Step Reagent for 20 minutes,
rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
- Incubate with INNOVEX substrate/chromogen of choice (AEC or DAB for HRP enzyme label) and
Innovex Brown, Fast Red, or other substrate/chromogen available for alkaline phosphatase enzyme
for 5-10 minutes, rinse with water;
- Counterstain and Mount.
For Immunostaining in 60 minutes
POLYMER HISTO-STAT staining systems are available in Complete Staining Systems and in
Individual Components
HISTO-STAT, No-wash, No-background Complete Staining Systems include all staining components required
including substrate/ chromogen of choice.
HISTO-STAT Complete Staining Systems are available in:
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HISTO-STAT Peroxidase Multivalent Complete System
(Large Volume)
(single staining system for staining both mouse and rabbit primary antibodies) includes:
• 50 ml of Peroxidase Second Step Reagent for staining both mouse and
rabbit primary antibodies
• 70 ml of stable AEC or DAB of choice.
Large Volume Stains 600-800 slides
HISTO-STAT Peroxidase Multivalent Complete System
(Small Volume)
(single staining system for staining both mouse and rabbit primary antibodies) includes:
• 20 ml of Peroxidase Second Step Reagent for staining both mouse and
rabbit primary antibodies
• 30 ml of stable AEC or DAB of choice.
Small Volume Stains 200-250 slides
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TYPE |
NUMBER |
PRICE |
|
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Multivalent-DAB
(LARGE VOLUME) |
NB312LD |
$785 |
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Multivalent-AEC
(LARGE VOLUME) |
NB312LC |
$785 |
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Multivalent-DAB
(SMALL VOLUME) |
NB312LD-20 |
$475 |
|
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Multivalent-AEC
(SMALL VOLUME) |
NB312LC-20 |
$475 |
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HISTO- STAT Alkaline phosphatase Multivalent Complete systems
(Large Volume)
(single system for staining both mouse and rabbit primary antibodies) include:
• 50 ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit
primary antibodies
• Substrate/chromogen of choice, Fast Red (70ml) or Innovex Brown (30ml)
Large Volume Stains 600-800 slides
HISTO- STAT Alkaline phosphatase Multivalent Complete systems
(Small Volume)
• 20 ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit
primary antibodies
• Substrate/chromogen of choice, Fast Red (70ml) or Innovex Brown (15ml)
Small Volume Stains 200-250 slides
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TYPE
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NUMBER
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PRICE
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Multivalent-FAST RED
(LARGE VOLUME)
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NB317LF
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$785
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Multivalent-INNOVEX BROWN
(LARGE VOLUME)
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NB317LB
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$785
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Multivalent-FAST RED
(SMALL VOLUME)
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NB317LF-20
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$495
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Multivalent-INNOVEX BROWN
(SMALL VOLUME)
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NB317LB-20
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$495
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COMPONENTS
HISTO-STAT staining system components are available in:
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| Item# |
Product Description |
Unit |
PriceUs $ |
Buy |
| NB312L |
HISTO-STAT Multivalent Second Step Reagent (HRP) for staining both MOUSE & RABBIT primary antibodies |
50 ml
|
$ 455
|
|
| NB312L-15 |
HISTO-STAT Multivalent Second Step Reagent (HRP) for staining both MOUSE & RABBIT primary antibodies |
20 ml
|
$ 325
|
|
| NB317L-15 |
HISTO-STAT Multivalent Second Step Reagent (Alkaline phosphatase) for staining both MOUSE & RABBIT primary antibodies |
20 ml
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$ 395
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| NB312C-15 |
HISTO-STAT Monovalent-Chicken Second Step Reagent (HRP) for staining CHICKEN primary antibodies |
15 ml
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$ 295
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| NB312M-15 |
HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP) for staining MOUSE primary antibodies |
15 ml
|
$ 295
|
|
| NB312R-15 |
HISTO-STAT Monovalent-Rabbit Second Step Reagent (HRP) for staining RABBIT primary antibodies |
15 ml
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$ 295
|
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| NB312S-15 |
HISTO-STAT Monovalent-Sheep Second Step Reagent (HRP) for staining SHEEP primary antibodies |
15 ml
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$ 295
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| NB312G-15 |
HISTO-STAT Monovalent-Goat Second Step Reagent (HRP) for staining GOAT primary antibodiess |
15 ml
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$ 295
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| NB312H-15 |
HISTO-STAT Monovalent-Hamster Second Step Reagent (HRP) for staining HAMSTER primary antibodies |
15 ml
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$ 295
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| NB312T-15 |
HISTO-STAT Monovalent-Rat Second Step Reagent (HRP) for staining Rat primary antibodies |
15 ml
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$ 295
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| NB312MRA-15 |
HISTO-STAT Monovalent-Rat Second Step Reagent (HRP) MOUSE ADSORBED for staining RAT primary antibodies in Mouse tissue |
15 ml
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$ 395
|
|
| NB312RMA-15 |
HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP) RAT ADSORBED for staining MOUSE primary antibodies in Rat tissue |
15 ml
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$ 395
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| NB317L |
HISTO-STAT Multivalent Second Step Reagent (Alkaline phosphatase) for staining both MOUSE & RABBIT primary antibodies |
50 ml
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$ 575
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