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For immunostaining in 50 minutes
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PAN CYTOKERATIN
HUMAN BREAST CARCINOMA |
bcl-2 |
CYTOKERATIN 7
HUMAN BREAST CARCINOMA |
CD 45
HUMAN TONSIL |
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For Wash free, Background free STAT (rapid) immunostaining
in 2 easy steps and under one hour
Free of avidin, biotin, and strepavidin
A single kit system for animal and human tissue staining
About HISTO-STAT
HISTO-STAT (2-step staining system), a Non-Biotin & Non-strepavidin system developed for Wash Free, Background Free, Pre-treatment Free immunostaining in 50 minutes.
This futuristic staining system involves only 2 staining steps post incubation with the primary antibody; an incubation with the SECOND STEP REAGENT and, a short incubation step (5 minutes) with the SUBSTRATE/CHROMOGEN of choice. HISTO-STAT system involves ONE LESS REAGENT, ONE LESS INCUBATION STEP and ONE LESS RINSE STEP.
UNIQUE FEATURES
• No Background or serum blocking step required
• Total staining time of only 50 minutes (from primary to mounted slides)
• Wash Free
• Background Free, no serum or protein blocking required
• Free of biotin; No avidin blocking required
• Short incubation steps
• 3 years of shelf life
• Highly amplified, equates the sensitivity of
biotin-strepavidin system
• No re-titration of current primary antibodies required, simply switch to
HISTO-STAT detection system
HISTO-STAT USER FRIENDLY PROTOCOL
TOTAL STAINING TIME: 50 MINUTES FOR PARAFFIN SECTION, 20 MINUTES FOR FROZEN SECTIONS
No protein blocking step or extensive rinses are required when staining with Innovex staining systems.
Post Deparaffinization:
1. Incubate the section or smear for 20 minutes with appropriate primary antibody, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
2. Incubate with the Peroxidase
(HRP) or alkaline phosphatase Second Step Reagent for 20 minutes, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
3. Incubate with INNOVEX substrate/chromogen of choice
(AEC or DAB for HRP enzyme label) and Innovex Brown, Fast Red, or other substrate/chromogen available for alkaline phosphatase enzyme for 5-10 minutes, rinse with water;
4. Counterstain and Mount.
For immunostaining in 50 minutes
HISTO-STAT staining systems are available in
COMPLETE STAINING SYSTEMS and in Individual COMPONENTS
HISTO-STAT, No-wash, No-background Complete Staining Systems include all staining components required including substrate/ chromogen of choice.
HISTO-STAT Complete Staining Systems are available in:
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HISTO-STAT Peroxidase Multivalent Complete System
(Large Volume)
(single staining system for staining both mouse and rabbit primary antibodies) includes:
• 50 ml of Peroxidase Second Step Reagent for staining both mouse and
rabbit primary antibodies
• 70 ml of stable AEC or DAB of choice.
Large Volume Stains 600-800 slides
HISTO-STAT Peroxidase Multivalent Complete System
(Small Volume)
(single staining system for staining both mouse and rabbit primary antibodies) includes:
• 20 ml of Peroxidase Second Step Reagent for staining both mouse and
rabbit primary antibodies
• 30 ml of stable AEC or DAB of choice.
Small Volume Stains 200-250 slides
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TYPE |
NUMBER |
PRICE |
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Multivalent-DAB
(LARGE VOLUME) |
NB312LD |
$785 |
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Multivalent-AEC
(LARGE VOLUME) |
NB312LC |
$785 |
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Multivalent-DAB
(SMALL VOLUME) |
NB312LD-20 |
$475 |
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Multivalent-AEC
(SMALL VOLUME) |
NB312LC-20 |
$475 |
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HISTO- STAT Alkaline phosphatase Multivalent Complete systems
(Large Volume)
(single system for staining both mouse and rabbit primary antibodies) include:
• 50 ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit
primary antibodies
• Substrate/chromogen of choice, Fast Red (70ml) or Innovex Brown (30ml)
Large Volume Stains 600-800 slides
HISTO- STAT Alkaline phosphatase Multivalent Complete systems
(Small Volume)
• 20 ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit
primary antibodies
• Substrate/chromogen of choice, Fast Red (70ml) or Innovex Brown (15ml)
Small Volume Stains 200-250 slides
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TYPE
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NUMBER
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PRICE
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Multivalent-FAST RED
(LARGE VOLUME)
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NB317LF
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$785
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Multivalent-INNOVEX BROWN
(LARGE VOLUME)
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NB317LB
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$785
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Multivalent-FAST RED
(SMALL VOLUME)
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NB317LF-20
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$495
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Multivalent-INNOVEX BROWN
(SMALL VOLUME)
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NB317LB-20
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$495
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COMPONENTS
HISTO-STAT staining system components are available in:
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| Item# |
Product Description |
Unit |
PriceUs $ |
Buy |
| NB312L |
HISTO-STAT Multivalent Second Step Reagent (HRP) for staining both MOUSE & RABBIT primary antibodies |
50 ml
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$ 455
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| NB312L-15 |
HISTO-STAT Multivalent Second Step Reagent (HRP) for staining both MOUSE & RABBIT primary antibodies |
15 ml
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$ 325
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| NB317L-15 |
HISTO-STAT Multivalent Second Step Reagent (Alkaline phosphatase) for staining both MOUSE & RABBIT primary antibodies |
15 ml
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$ 395
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| NB312C-15 |
HISTO-STAT Monovalent-Chicken Second Step Reagent (HRP) for staining CHICKEN primary antibodies |
15 ml
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$ 295
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| NB312M-15 |
HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP) for staining MOUSE primary antibodies |
15 ml
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$ 295
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| NB312R-15 |
HISTO-STAT Monovalent-Rabbit Second Step Reagent (HRP) for staining RABBIT primary antibodies |
15 ml
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$ 295
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| NB312S-15 |
HISTO-STAT Monovalent-Sheep Second Step Reagent (HRP) for staining SHEEP primary antibodies |
15 ml
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$ 295
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| NB312G-15 |
HISTO-STAT Monovalent-Goat Second Step Reagent (HRP) for staining GOAT primary antibodiess |
15 ml
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$ 295
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| NB312H-15 |
HISTO-STAT Monovalent-Hamster Second Step Reagent (HRP) for staining HAMSTER primary antibodies |
15 ml
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$ 295
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| NB312T-15 |
HISTO-STAT Monovalent-Rat Second Step Reagent (HRP) for staining Rat primary antibodies |
15 ml
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$ 295
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| NB312MRA-15 |
HISTO-STAT Monovalent-Rat Second Step Reagent (HRP) MOUSE ADSORBED for staining RAT primary antibodies in Mouse tissue |
15 ml
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$ 395
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| NB312RMA-15 |
HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP) RAT ADSORBED for staining MOUSE primary antibodies in Rat tissue |
15 ml
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$ 395
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| NB317L |
HISTO-STAT Multivalent Second Step Reagent (Alkaline phosphatase) for staining both MOUSE & RABBIT primary antibodies |
50 ml
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$ 575
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