For
immunostaining in 50 minutes
|
bcl-2 |
PCNA |
PAN CYTOKERATIN |
LAMBDA |
 |
 |
|
 |
For
Wash free, Background free STAT (rapid) immunostaining
in 2 easy steps and under one hour
Free of avidin, biotin, and strepavidin
A single kit system for animal and human tissue staining
About HISTO-STAT
HISTO-STAT (2-step staining system), a Non-Biotin
& Non-strepavidin system developed for Wash Free, Background
Free, Pre-treatment Free immunostaining in 50 minutes.
This
futuristic staining system involves only 2 staining steps
post incubation with the primary antibody; an incubation with
the SECOND STEP REAGENT and, a short incubation step (5 minutes)
with the SUBSTRATE/CHROMOGEN of choice. HISTO-STAT system
involves ONE LESS REAGENT, ONE LESS INCUBATION STEP and ONE
LESS RINSE STEP.
UNIQUE FEATURES
•
No Background or serum blocking step required
• Total staining time of only 50 minutes (from primary
to mounted slides)
• Wash Free
• Background Free, no serum or protein blocking required
• Free of biotin; No avidin blocking required
• Short incubation steps
• 3 years of shelf life
• Highly amplified, equates the sensitivity of biotin-strepavidin
system
• No re-titration of current primary antibodies required,
simply switch to HISTO-STAT detection system
HISTO-STAT USER FRIENDLY PROTOCOL
TOTAL STAINING TIME: 50 MINUTES FOR PARAFFIN SECTION, 20 MINUTES
FOR FROZEN SECTIONS
No protein blocking step or extensive rinses are required
when staining with Innovex staining systems.
Post Deparaffinization:
1. Incubate the section or smear for 20 minutes with appropriate
primary antibody, rinse once with PBS or Innovex “Signal
Enhancing Wash Buffer” for 5 seconds;
2. Incubate with the Peroxidase (HRP) or alkaline phosphatase
Second Step Reagent for 20 minutes, rinse once with PBS or
Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
3.
Incubate with INNOVEX substrate/chromogen of choice (AEC or
DAB for HRP enzyme label) and Innovex Brown, Fast Red, or
other substrate/chromogen available for alkaline phosphatase
enzyme for 5-10 minutes, rinse with water;
4. Counterstain and Mount.
For
immunostaining in 50 minutes
HISTO-STAT staining systems are available
in
COMPLETE STAINING SYSTEMS and in Individual
COMPONENTS
HISTO-STAT, No-wash, No-background Complete Staining Systems
include all staining components required including substrate/
chromogen of choice. HISTO-STAT
Complete Staining Systems are available in:
HISTO-STAT
Peroxidase Multivalent Complete System (Large
Volume)
(single staining system for staining both mouse and
rabbit primary antibodies) includes:
• 50 ml of Peroxidase Second Step Reagent for
staining both mouse and
rabbit primary antibodies
• 70 ml of stable AEC or DAB of choice.
Large Volume Stains 600-800 slides HISTO-STAT
Peroxidase Multivalent Complete System (Small
Volume)
(single staining system for staining both mouse and
rabbit primary antibodies) includes:
• 20 ml of Peroxidase Second Step Reagent for
staining both mouse and
rabbit primary antibodies
• 30 ml of stable AEC or DAB of choice.
Small Volume Stains 200-250 slides
TYPE
|
NUMBER
|
PRICE |
|
Multivalent-DAB
(LARGE VOLUME) |
NB312LD |
$695 |
|
Multivalent-AEC
(LARGE VOLUME) |
NB312LC |
$695 |
|
Multivalent-DAB
(SMALL VOLUME) |
NB312LD-20 |
$395 |
|
Multivalent-AEC
(SMALL VOLUME) |
NB312LC-20 |
$395 |
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HISTO-
STAT Alkaline phosphatase Multivalent Complete systems
(Large
Volume)
(single system for staining both mouse and
rabbit primary antibodies) include:
• 50 ml of alkaline phosphatase Second Step Reagent
for staining both mouse and rabbit
primary antibodies
• Substrate/chromogen of choice, Fast Red (70ml)
or Innovex Brown (30ml)
Large Volume Stains 600-800 slides HISTO-
STAT Alkaline phosphatase Multivalent Complete systems
(Small
Volume)
• 20 ml of alkaline phosphatase Second Step Reagent
for staining both mouse and rabbit
primary antibodies
• Substrate/chromogen of choice, Fast Red (70ml)
or Innovex Brown (15ml)
Small Volume Stains 200-250 slides
TYPE
|
NUMBER
|
PRICE |
|
Multivalent-FAST
RED
(LARGE VOLUME) |
NB317LF |
$785 |
|
Multivalent-INNOVEX
BROWN
(LARGE VOLUME) |
NB317LB |
$695 |
|
Multivalent-FAST
RED
(SMALL VOLUME) |
NB317LF-20 |
$495 |
|
Multivalent-INNOVEX
BROWN
(SMALL VOLUME) |
NB317LB-20 |
$395 |
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COMPONENTS
HISTO-STAT staining system components are available
in:
|
|
Item# |
Product Description |
Unit |
PriceUs $ |
Buy |
|
NB312L |
HISTO-STAT Multivalent Second Step Reagent (HRP) for
staining both MOUSE & RABBIT primary antibodies |
50 ml |
$
395 |
|
|
NB312L-15 |
HISTO-STAT Multivalent Second Step Reagent (HRP) for
staining both MOUSE & RABBIT primary antibodies |
15 ml |
$
245 |
|
|
NB317L-15 |
HISTO-STAT Multivalent Second Step Reagent (Alkaline
phosphatase) for staining both MOUSE & RABBIT primary
antibodies |
15 ml |
$
245 |
|
|
NB312C-15 |
HISTO-STAT Monovalent-Chicken Second Step Reagent (HRP)
for staining CHICKEN primary antibodies |
15 ml |
$
245 |
|
|
NB312M-15 |
HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP)
for staining MOUSE primary antibodies |
15 ml |
$
245 |
|
|
NB312R-15 |
HISTO-STAT Monovalent-Rabbit Second Step Reagent (HRP)
for staining RABBIT primary antibodies |
15 ml |
$
245 |
|
|
NB312S-15 |
HISTO-STAT Monovalent-Sheep Second Step Reagent (HRP)
for staining SHEEP primary antibodies |
15 ml |
$
245 |
|
|
NB312G-15 |
HISTO-STAT Monovalent-Goat Second Step Reagent (HRP)
for staining GOAT primary antibodiess |
15 ml |
$
245 |
|
|
NB312H-15 |
HISTO-STAT Monovalent-Hamster Second Step Reagent (HRP)
for staining HAMSTER primary antibodies |
15 ml |
$
245 |
|
|
NB312T-15 |
HISTO-STAT Monovalent-Rat Second Step Reagent (HRP)
for staining Rat primary antibodies |
15 ml |
$
245 |
|
|
NB312MRA-15 |
HISTO-STAT Monovalent-Rat Second Step Reagent (HRP)
MOUSE ADSORBED for staining RAT primary antibodies in
Mouse tissue |
15 ml |
$
245 |
|
|
NB312RMA-15 |
HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP)
RAT ADSORBED for staining MOUSE primary antibodies in
Rat tissue |
15 ml |
$
245 |
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